Figure 4.

KLK6 mediates the tumorigenicity and stemness of the HGC-27 cells in vivo. (A) sh-KLK6 attenuated the tumorigenicity and KLK6 mRNA expression level of the HGC-27 cells in vivo. (B) KLK6 silencing inhibited the levels of ATP, lactic acid, glucose and the protein expression levels of HK1, HK2, and GLUT1 in vivo, and the levels of Nanog and Oct-4 were detected using immunohistochemistry. (C) Effect of KLK6 on the proteins involved in the PI3K/AKT/mTOR signaling pathway was detected using western blot analysis. *P<0.05 vs. control. n=3. OV, overexpression; NC, negative control; sh, short hairpin; KLK6, kallikrein 6.