Figure 1.
Uptake of α-syn fibrils results in the induction of an inflammatory profile
(A) Quantification of the percentage of phagocytic cells (left) and the individual uptake index per cell (middle) after exposure to fluorescent α-syn fibrils (2 μM); n = 4. Diagram represents the α-syn uptake as measured by FACS (right).
(B) Representative immunostaining showing the internalization of α-syn fibrils into CD11b+ microglia.
(C) Heatmap of 2189 differentially expressed (DE) genes between control and α-syn-treated microglia.
(D) Top 15 DE genes belonging to the α-syn signature identified by (Sarkar et al., 2020) in murine microglia plotted as Z-score transformed heatmap of gene expression values.
(E) Bar chart of most enriched pathways for aggregated α-syn induced (red) and suppressed (blue) genes.
(F) BiNGO enrichment map for DE genes between control and α-syn-treated microglia. Clusters were defined by the Cytoscape tool Wordcloud.
FC, fold change; ES, enrichment score.
Graphs represent the mean ± SEM and were analyzed by one-way ANOVA followed by Tukey’s multiple comparison post hoc test. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05.