Figure 6.
Cell-to-cell transfer of aggregated α-syn in microglia in vivo
(A) Schematic illustrating the preparation of organotypic slice cultures (OSC) used for experiments shown in (B) and (C).
(B and C) Representative immunostainings and 3D reconstructions of CellTracer labeled microglia containing α-syn injected into the cortex (B) or hippocampus (C) of an OSC connected to tissue-resident microglia with α-syn-positive inclusions.
(D) Schematic illustrating in vivo 2-photon imaging used for experiments shown in (E) and (F).
(E) Representative recording demonstrating the formation of a microglial network (Cx3cr1GFP) upon the injection of α-syn.
(F) Representative time-lapse recordings demonstrating the transfer of α-syn between microglia via cellular membrane connections. Cellular connections were retracted (asterisk) once α-syn got transferred to the neighboring microglia.
Schematics were created using BioRender.com.
Scale bars: 20 μm. See also Figure S7.