Figure S8.

α-syn aggregates trigger a stronger ROS release in DLB patient-derived monocyte-derived microglia, related to Figure 7

(A) Representative immunohistochemical staining (left panel) and 3D reconstruction (right panel) of human cingulate gyrus samples from MSA patients. Samples were analyzed for Iba1-positive microglia (white) and α-syn (red). DAPI (blue) was used as nuclear counterstain.

(B) Representative immunostaining (left panel) and 3D reconstruction (right panel) of human cingulate gyrus tissues from MSA patients. Samples were analyzed for Iba1-positive microglia (white) and α-syn (red).

(C) Representative super-resolution imaging of human cortical tissues from DLB patients. Samples were analyzed for Iba1-positive microglia (red) and α-syn (red).

(D) Schematic drawing of the use of patient monocytes-derived microglia.

(E and F) Quantification of the mean CellROX signal (E) and the percentage of CellROX positive cells (F) using patient-derived monocyte-derived microglia treated for 24 h with 1 μM α-syn fibrils.

All graphs are presented as mean ± SEM and were analyzed by a two-way ANOVA in conjunction with Sidak’s multiple comparison test. ^∗p < 0.05.

Scale bar: 20 μm.