Supplementary Figure 8.

Exploratory correlation matrices of calprotectin, microbiome, microscopic, and BA data in IBS and UC. Correlation matrices with Pearson correlation coefficients for patients with IBS (top panel) and patients with UC (bottom panel). Microscopic data from colonic biopsies (average and maximum width of methacarn-fixed PAS-stained surface layer on top of epithelium per section [average PAS layer height, maximum PAS layer height], maximum width of methacarn-fixed H&E surface layer on top of epithelium per section [surface layer], total number of bacteria normalized to length of epithelium per section [amount of bacteria], maximum density of bacteria in one 144.7 × 144.7 μm confocal microscopy image per section [bacterial density], number of bacteria 3 μm from the epithelium, normalized to length of epithelium per section [adherent bacteria], maximum density of bacteria up to 3 μm distal from the epithelium in one 144 × 144 μm confocal microscopy image per section [adherent bacteria density]), ratio of quantitative polymerase chain reaction determined 16S rRNA gene copies to total DNA of biopsies (DNA ratio), microbiome data from colonic biopsies (relative abundance of OTU_tq6_57b belonging to the Faecalibacterium genus and OTU_9qp_ahy belonging to the Escherichia/Shigella genus, microbiome richness and Shannon diversity), BA data from stool samples (total BA, cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, and UCDA), calprotectin data from stool samples (calprotectin). Positive correlation coefficients in blue, negative correlation coefficients in red. P values ≤.05 have been colored. (A) n = 61. (B) n = 30.