Fig. 2.

OGR1 inhibitor reduces macroscopic disease indicators of intestinal inflammation in acute DSS-induced colitis. Endoscopy was performed, and images were scored according to the MEICS criteria (a, b). Representative pictures are shown (a). Colon specimens were stained with hematoxylin and eosin (c); epithelial damage and leukocyte infiltration scores were assessed in the distal colon (d). Representative pictures are shown (c). Scale bars, 200 and 500 μm. Results are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by the post hoc Tukey's test. Water/vehicle (methylcellulose 0.5%) (n = 7), vehicle/DSS (n = 7), and OGR1 inhibitor/DSS: 25, 50, and 100 mg/kg (n = 6, 7, and 7, respectively). *p < 0.05, **p < 0.01, and ***p < 0.001. OGR1, ovarian cancer G protein-coupled receptor 1; MEICS, Murine Endoscopic Index of Colitis Severity; DSS, dextran sulfate sodium.