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. 2021 Oct 20;41(8):1709–1722. doi: 10.1007/s10875-021-01133-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Gating strategy to identify S + and S + + MBCs, PBs, ATM , and activated MBCs. One HD and two CVID subjects are shown. We analyzed CD19 + B-cells, included in the live gate. SA PE-Cy7 was used as a decoy probe to gate out streptavidin-binding B-cells from further analysis. MBCs were identified as CD24 + CD27 + CD38-; PBs as CD24-CD27 + + CD38 + + ; ATMs as CD24-CD27-CD38-CD21- -; and activated MBCs as CD24- CD38-CD21-CD27 + . Flow cytometry plots show the staining patterns of SARS-CoV-2 antigen probes in the indicated B-cell populations during the follow-up. S + are B-cells that are Spike-PE + , but Spike-BUV395-. S + + are instead Spike-PE + and SpikeBUV395 + . The color code identifies Spike + and + + in MBCs (blue), PBs (orange), ATMs (dark red) ), and Activated MBCs (green)