Fig. 3.

Peripheral blood B-cells subsets, low and high binding capacity B-cells for recombinant Spike protein in HD (blue circles) and in CVID (green circles) before (T0, dark color circles) and after two doses of the BNT162b2 vaccine (T1, lighter color circles). We show the frequencies of peripheral blood MBCs (panel a), Activated MBCs (b), ATMs (c), and PBs (e) in HD (blue circles) and CVID (green circles). The frequency of S + and S +  +  B-cells inside each identified B-cell population is shown (e, f, g, and h). Medians are plotted as horizontal bars and statistical significance were determined using two-tailed Mann–Whitney U-test or Wilcoxon matched-pairs signed-rank test. *P < 0.05, **P < 0.01, ***P < 0.001; ****P < 0.0001. N = 28 HD and N = 26 CVID patients. B-cells subsets were defined as following: MBCs CD19 + CD24 + CD27 + CD38-; activated MBCs CD19 + , CD27 + CD24-CD38-; specific ATMs CD19 + CD27-CD24-CD28-; PBs CD19 + CD24-CD38 +  + CD27 +  +