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. 2020 Dec 16;104(3):717–730. doi: 10.1093/biolre/ioaa225

Figure 5 .

Figure 5

HN and IL-27Rα or EBI-3 interaction detected by dot blot. (A) The peptides used for dot blots included: humanin (HN 1, 2, and 5 nmol), SP (1, 2, and 5 nmol, peptide with the same amino acid as HN but scrambled was used as negative control), BAX (1, 2, and 5 mcg, positive control), IL-27Rα (1, 2, and 5 mcg), and human IgG Fc (1, 2, and 5 mcg, negative control for IL-27Rα). Dot blots confirmed the interaction between HN and IL-27Rα. (B) The peptides used for dot blots included: humanin (HN 1, 2, and 5 nmol), SP (1, 2, and 5 nmol, negative control), BAX (0.1, 1, and 5 mcg, positive control), EBI-3 (0.1, 1, and 5 mcg), p28 (0.1, 1, and 5 mcg), p35 (0.1, 1, and 5 mcg), p40 (0.1, 1, and 5 mcg), and human IgG Fc (0.1, 1, and 5 mcg, negative control for EBI-3). Dot blots confirmed the specific interaction between HN and EBI-3.