FIG. 12.

The P3HR1 strain also prevents apoptosis and allows cell division in Ramos cells treated with cisplatin (10 μg/ml). (A) A Western blot showed that although Ramos cells were sensitive to cisplatin-induced apoptosis as measured by PARP cleavage, two independent sublines of Ramos infected with the P3HR1 strain of EBV (AW- and EHRB-Ramos) were protected over the same 16-h time course. (B) After exposure to cisplatin, Ramos cells fail to complete mitosis [compare 16h Ramos (+ cisplatin) and (− cisplatin)], whereas the P3HR1 EBV-infected EHRB-Ramos cell line is able to complete mitosis [EHRB (+ cisplatin); the asterisk indicates the labeled G1 cells]. (C) Cell cycle profiles of BrdU-labeled populations were electronically gated as described for Fig. 2.