Fig. 4. (A) Product yields after 180 min for background reactions of DNA donor conjugates 30a or 30b with acceptor conjugates 23a –23c. (B–E) Fold change of product formation upon introduction of unpaired template nucleotides (underlined in B) relative to seamless annealing (0 nt) for transfer reactions of DNA donor conjugates 30a or 30b with DNA acceptor conjugate 23b (C), DNA acceptor conjugate 23c (D), or PNA acceptor conjugate 23a (E). Error bars show the standard deviation of triplicate measurements. For details, see Tables S3–S6.† Mean yields given in numbers over the bars. (F) Product yields after 720 min for the indicated transfer reactions in presence and absence of RNA template. Conditions: 10 mM MOPS, 200 mM NaCl, 2 mM TCEP, pH 7.2, [A] = 1 μM, [D] = 2 μM, [RNA] = 0/1 μM, 37 °C. BG = background; nt = nucleotides.