TABLE 1.
Cell trafficking of 125I‐BMP2 in C2C12 cells
| Total Cell Associated BMP2 | |
| Cell Surface % | 19.68 + 0.72 |
| Internalized % | 80.32 + 0.72 |
| Recycled BMP2 | |
| Total Recycled, % Internalized | 40.36 + 0.50 |
| Cell Surface % | 13.78 + 0.57 |
| Released to Media % | 86.22 + 0.57 |
| Recycled Media BMP2 | |
| EV fraction % | 82.23 + 0.58 |
| “Free” BMP2 fraction % | 13.54 + 0.56 |
| Recycled Media BMP2 EV, cargo location | |
| EV surface % | 92.01 + 0.77 |
| EV lumen % | 7.99 + 0.77 |
Data represent the mean + SEM of 6 replicate cultures of a representative experiment for total cell associated BMP2 and recycled BMP2. Whereas data represents the mean + SEM of 3 experiments conducted using ‘pooled’ recycled media BMP2.