. 2021 Oct 20;9(2):e01123-21. doi: 10.1128/Spectrum.01123-21

TABLE 3.

Amino acid similarities of products of predicted Serratia variable TcA and TcC orthologs to translated amino acid sequences in the database detected using BLASTP

ORF	No. amino acid residues	Isolates^a	Locus tag	Species (amino acid length [bp])	Area of amino acid similarity^b	Amino acid residues of target sequence	Ortholog accession no.
TcA
TcA1, SepA	2,367	A1MO2, IDIA	SppA	S. proteamaculans (2,406)	(62/73)/2,443	1–2406	ACZ05627.1
TcA2, SppA	2,406	143, Cfb	SepA	S. entomophila (2,376)	(62/73)/2,443	6–2376	WP_010895734.1
			Toxin complex TcYF1	Yersinia frederiksenii (2,363)	(70/79)/2,426	6–2363	AAP48861.2
TcA3	2,499	R10, K, M, 28F, 20E, 12D, 25E, G, 10NOV	SppA	S. proteamaculans (2,406)	(90/92)/1,198	1–1198	ACZ05627.1
			Toxin complex	Xenorhabdus bovienii (2,506)	(44/59)/2,549	21–2506	WP_051861318.1
TcA4	2,409	Man4, C	SppA	S. proteamaculans (2,406)	(94/95)/2,406	1–2406	ACZ05627.1
TcC^c
TcC1, SepC	973	NA	Rhs repeat protein	Salmonella enterica (985)	(78/85)/985	1–985	EBR4566301.1
TcC2a, SppC	964	C1 (R10, 20E, Cfb, 143, 28F); C2 (12D, Rm5)	TcC component	Enterobacter sp. (956)	(66/77)/963	1–950	BBF83683.1
TcC2b	952	C1 (C, Man4)	SppC	S. proteamaculans (952)	(94/96)/952	1–952	ABB69941.1
TcC3	970	C2 (C, Man4)	Type IV secretion protein Rhs	Pantoea wallisii (977)	(66/77)/988	1–977	ORM74714.1
			SppC	S. proteamaculans (952)	(95/97)/680	1–680	ABB69941.1
TcC4	1,019	C1 (RM5, M, K, 12D, 25E)	Insecticidal toxin	Xenorhabdus ishibashii (1,020)	(54/67)/1,031	1–1020	PHM61563.1
			SppC	S. proteamaculans (952)	(98/98)/697	1–679	ABB69941.1

Refer to Table 1 for isolate source.

Area of amino acid similarity (% identity/% similarity over the indicated range of amino acid residues) in relation to sequence generated in this study. Similarities were considered significant if the BLASTP score exceeded e⁻⁵. Refer to Figure 4 for schematic of virulence clusters.

C1 and C2 denote respective TcC operon components. NA, not available.