FIG 4.

Distribution of the increase in the frequency of parasites with mutant pfcrt 76 alleles between the gametocyte and sporozoite stages for experimental infections where the proportion of parasites harboring the considered allele did not exceed 20% at the gametocyte stage. The comparison of the proportion of mutant pfcrt 76 alleles was restricted to experimental feedings where gametocyte samples had a proportion of mutant pfcrt alleles below 20%, defining these parasites as a minority variant. The difference of proportions between the gametocyte and sporozoite stages was computed, where a positive value meant an increase in the proportion of parasites with the mutant pfcrt allele at the sporozoite stage. The dashed line represents the average of this increase.