FIG 5.
SCFAs promote adenovirus replication in vitro. HAdV-positive lymphocytes were isolated and cultured. The lymphocytes were seeded at 1 × 106 cells/well on 12-well plates. The cells were treated with different concentrations of sodium acetate (A), sodium butyrate (B), sodium isobutyrate (C), sodium isovalerate (D), sodium propionate (E), LPS (F), TNF-α (G), and IL-6 (H) for 24 h. The supernatant was taken and treated with proteinase K to determine the levels of the HAdV early gene E3 and E4 DNA by qPCR.