FIG 4.

Enhanced resistance of AHR^−/− THP-1 cells to HSV-1 infection is independent of IFN-β. WT THP-1 cells and AHR^−/− THP-1 cells were pretreated with α-NF for 24 h, and IFN-β antibodies (Abs) or isotype antibody was added at the same time as HSV-1 infection for 24 h. (A) Percentages of GFP⁺ cells were analyzed by flow cytometry. (B) Percentages of GFP-positive cells are shown in a histogram. The mRNA of the IFN-inducible genes IFIT1 and OAS1 was measured by qPCR. (C) Viral titers in each group were determined by a TCID₅₀ assay. *, P < 0.05; **, P < 0.01. Data are shown as means ± SEM.