FIG 2.

(A) Zymogram of isolated crude autolysin extract of all strains (volume adjusted to represent a similar amount of cells for all strains based on OD₆₀₀) tested against M. luteus cells. Different fragments of the major autolysin AtlA are annotated (PP, propeptide; AM, amidase subunit with two repeat domains; GL, glucosaminidase subunit with one repeat domain). The figure shows a representative experiment; the zymogram was repeated three times. (B) Lysis assay measured after 2 hours of incubation with crude autolysin extract of each strain (volume adjusted to represent a similar amount of cells for all strains based on OD₆₀₀) and purified PGN with WTA without d-alanine substitutions of each strain (*, P < 0.05; ***, P < 0.0005).