TABLE 4.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Staphylococcus aureus | ||
| NCTC8325 | Laboratory strain, MSSA, agr+ | 61 |
| RN4220 | Transformable laboratory strain, MSSA, agr− | 27 |
| RN4220ΔmutS | mutS knockout mutant of S. aureus RN4220, mutator phenotype, agr− | 18 |
| VC40 | Vancomycin-resistant mutant of S. aureus RN4220ΔmutS, agr+ | 6 |
| VC40R | Spontaneous revertant of S. aureus VC40 with additional exchange (M54T) in VraR, agr+ | This study |
| VraS(VC40) | Derivative of strain S. aureus NCTC8325 harboring the D242G and L114S exchanges of S. aureus VC40 in VraS, agr+ | 6 |
| RN4220ΔtarMΔtarS | Deletion mutant for both WTA glycosylases of S. aureus RN4220, agr− | 62 |
| Escherichia coli | ||
| JM109 | E. coli cloning host | 63 |
| DC10B | Universal staphylococcal cloning host | 64 |
| M15 | Strain for recombinant gene expression | 65 |
| BL21(DE3) pREPgroESL | Strain for recombinant gene expression, λDE3 lysogen, T7 RNA polymerase gene, constitutive expression of chaperons | 49 |
| Micrococcus luteus | ||
| ATCC 4698 | Sensitive indicator organism | ATCC |
| Plasmids | ||
| pTX15 | Xylose-inducible staphylococcal expression vector, Tetr | 66 |
| pTtarS | pTX15 plasmid for xylose-inducible expression of tarS | This study |
| pQE-32_AtlA | Overexpression plasmid for AtlA without leader sequence | This study |
| pET22bΔpelB_VraR | Overexpression plasmid for VraR | This study |
| pET22bΔpelB_VraR(M54T) | Overexpression plasmid for VraR(M54T) | This study |
| pET22bΔpelB_VraS | Overexpression plasmid for VraS | This study |