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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nature. 2021 May 10;594(7864):553–559. doi: 10.1038/s41586-021-03594-0

Extended Data Figure 9. Mucosal SARS-CoV-2 IgG responses in bronchoalveolar lavage (BAL) and nasal wash fluids before and after SARS-CoV-2 challenge.

Extended Data Figure 9.

a ELISA binding titers for SARS-CoV-2-specific IgG in 10X BAL fluid from macaques immunized with (blue symbols, left column) RBD-scNP three times or S-2P mRNA-LNP twice and RBD-scNP once (red symbols, left column). Day -7 BAL fluid was collected at week 10 or 16 for the RBD-scNP alone group or the S-2P mRNA-LNP/RBD-scNP group respectively. Group mean±s.e.m. are shown (N=5).

b-d 10X BAL fluid blocking of ACE2, RBD neutralizing antibody DH1041, and cross-nAbDH1047 binding to SARS-CoV-2 D614G stabilized spike ectodomain. A black horizontal bar indicates the group mean blocking percentage. Blocking above 20% (above the dashed line) is considered positive.

e Neat nasal wash fluid from RBD-scNP-immunized or S-2P mRNA-LNP/RBD-scNP-immunized macaques. Day -7 nasal wash fluid was collected at week 16 and 2 and 4 days post challenge for the S-2P mRNA-LNP/RBD-scNP group. Nasal wash fluid was unavailable for the RBD-scNP before challenge, but was collected 2 and 4 days after the week 11 challenge. Group mean±s.e.m. are shown (N=5).