FIG. 2.

(A) Inhibition of binding of IGIV to GBS polysaccharide type Ia by homologous and heterologous GBS polysaccharides. IGIV (1/1,000 dilution) was incubated with GBS polysaccharide types Ia, II, and III at concentrations of 2.50 to 0.08 μg/ml. For homologous inhibition, 100 μl from the preincubated mixture of type Ia GBS and IGIV was transferred to GBS type Ia polysaccharide-coated Immulon 4 plates. For heterologous inhibition, 100 μl from the preincubated mixture of IGIV with GBS polysaccharide type II or III was transferred to GBS type Ia-coated Immulon 4 plates. (B) Homologous inhibition of binding of IGIV to GBS polysaccharide Ia, II, and III coated plates. IGIV (1/1,000 dilution) was incubated with GBS polysaccharides as described above and then transferred to GBS polysaccharide Ia-, II-, and III-coated plates, respectively. Inhibition ELISA was performed as described in the text.