FIG. 3.

Relative quantitation of polysaccharides elaborated by five different strains of type V GBS. The strains were grown in 5 ml of Todd-Hewitt liquid medium for 24 h, heat inactivated, and neutralized as described in Materials and Methods. One hundred microliters of IGIV (1:1,000 dilution) was incubated with serial dilutions of suspensions from different strains and 100 μl was transferred to GBS type V polysaccharide-coated plates for ELISA.