Table 4. Mutations in PCS mutants compared to the ΔprkAcond parent strain.
| Isolate | Locus | Name | Mutation | Variant | Function/Description |
|---|---|---|---|---|---|
| PCS1 | Lmrg_01600 | G2282263A | E337K | Hypothetical protein | |
| Lmrg_01695 | murZ | C2583617A | Intergenic (39 bp upstream of start) | MurA paralog | |
| PCS2 | Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase |
| Lmrg_01780 | clpP | G2496832A | R112H | Catalytic subunit of Clp protease | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS3 | Lmrg_02674 | clpC | C246587A | Q512K | ATPase subunit of Clp protease |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS6 | Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase |
| Lmrg_01695 | murZ | C2582699CT | Frameshift, premature stop codon | MurA paralog | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| Lmrg_01919 | C2822334A | Silent | Hypothetical protein | ||
| PCS7 | Lmrg_01780 | clpP | G2496732A | D79N | Catalytic subunit of Clp protease |
| PCS8 | Lmrg_00641 | cbiE | C1180142T | P96S | Precorrin-6B methylase |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01467 | reoM | A1491250AT | Frameshift, premature stop codon | Regulator of MurA degradation | |
| Lmrg_05020 | G1699725A | tRNA-Asn | |||
| Lmrg_01834 | sufD | C2440473T | D45N | iron-sulfur cluster assembly protein | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS14 | Lmrg_02674 | clpC | C245359G | Y102* | ATPase subunit of Clp protease |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS16 | Lmrg_02076 | C989513T | G133E | GNAT family n-acetyltransferase | |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01467 | reoM | C1491331T | Intergenic (12 bp upstream of start) | Regulator of MurA degradation | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS17 | Lmrg_02608 | gpmA | G284397T | Intergenic (19 bp upstream of start) | Phosphoglycerate mutase |
| Lmrg_01467 | reoM | A1491250AT | Frameshift, premature stop codon | Regulator of MurA degradation | |
| Lmrg_02829 | rplT | C1815571T | G23E | 50S ribosomal protein 20 | |
| PCS19 | Lmrg_00339 | C677634T | E70K | GNAT family n-acetyltransferase | |
| Lmrg_00367 | flhB | C695925T | Silent | Flagellar biosynthesis | |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01695 | murZ | C2582788T | G262E | MurZ paralog | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump | ||
| PCS20 | Lmrg_01467 | reoM | T1491349C | Intergenic (30 bp upstream of start) | Regulator of MurA degradation |
| PCS21 | Lmrg_02674 | clpC | C246587A | Q512K | ATPase subunit of Clp protease |
| Lmrg_00741 | oatA | C1277896T | Silent | Peptidoglycan o-acetyltransferase | |
| Lmrg_01761 | G2516839A | Silent | DUF-containing protein | ||
| Lmrg_01723 | mreB | G2557908GA | Frameshift, premature stop codon | Rod shape-determining protein | |
| Lmrg_01945 | G2789642A | A241T | ATP-binding subunit of multidrug efflux pump |
Shading represents SNPs in genes involved in the ReoM pathway characterized by Wamp et al. [52].