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Hop1 protein binds selectively to oligonucleotides containing contiguous G-rich residues. Reaction mixtures (20 μl) contained 20 mM Tris-HCl (pH 7.5), 0.1 mM ZnCl2, 10 pmol of 32P-labeled oligonucleotide (Table 1), and the specified concentration of Hop1 protein. Samples were incubated at 30°C, separated on a 6% polyacrylamide gel, and visualized by autoradiography.
