Table 1. List of primary and secondary antibodies.
IHC, immunohistochemistry; WB, Western blot; IgG, immunoglobulin G.
| Primary antibody | Reference | Application | Dilution |
Secondary
antibody |
Antibody origin | Dilution |
| Rabbit polyclonal to phospho-GluN2B (Y1472) |
Abcam, Cambridge, UK; ref.: ab3856 |
IHC | 1:200 | Alexa Fluor 488 goat anti-rabbit IgG |
Thermo Fisher Scientific, Waltham USA, ref.: A11034 |
1:500 |
| Rabbit monoclonal to phospho-Erk1/2 (Thr202/Tyr204) |
Cell Signaling Technology, Danvers, USA; ref.: 4370S |
IHC | 1:400 | Alexa Fluor 488 goat anti-rabbit IgG |
Thermo Fisher Scientific, ref.: A11034 |
1:500 |
| Rat monoclonal to D1R |
Sigma-Aldrich, St Louis, USA; ref.: D2944 |
PLA | Dual PLA: 1:200;single PLA: 1:200 |
|||
| WB | 1:500 | ECL goat anti-rat IgG, HRP-linked |
GE Healthcare, Chicago, USA; ref.: NA935 |
1:5000 | ||
| Rabbit polyclonal to NMDAR1 |
Abcam, Cambridge, UK; ref.: ab17345 |
PLA | Dual PLA: 1:200; single PLA: 1:400 |
|||
| Rabbit polyclonal to D2R |
Millipore, Burlington, USA; ref.: ABN462 |
PLA | Dual PLA: 1:200; single PLA: 1:400 |
|||
| WB | 1:500 | WB: ECL goat anti-rabbit IgG, HRP-linked |
GE Healthcare; ref.: NA934 |
1:5000 | ||
| Mouse monoclonal to NMDAR2B NT |
Millipore; ref.: MAB5782 |
PLA | Dual PLA: 1:200; single PLA: 1:500 |
|||
| WB | 1:500 | WB: ECL goat anti-mouse IgG, HRP-linked |
GE Healthcare, Chicago, USA; ref.: NA931 |
1:5000 | ||
| Mouse monoclonal to NMDAR1 CT |
Millipore; ref.: 05-432 |
WB | WB: 1:200 | WB: ECL goat anti-mouse IgG, HRP-linked |
GE Healthcare; ref.: NA931 |
1:5000 |
| Rabbit polyclonal to Fos B |
Santa Cruz Biotechnology; ref: sc-48 |
IHC | 1:500 | Cy5 goat anti-rabbit IgG |
Jackson ImmunoResearch; ref: AB_2338013 |
1:1000 |