Table 1.
Factors and Five Different Levels in CCD Design Experiment. HSA Concentration, pH Value, Volume of Ethanol and Speed of Ethanol Addition Were Selected for Multi-Factor Analysis to Obtain the Optimal Prescription for HSA Preparation. 5 Levels for Each Factors Were Designed by CCD-Response Surface Methodology (CCD-RSM)
| Factors | Symbol | Coded Levels | ||||
|---|---|---|---|---|---|---|
| −2 | −1 | 0 | 1 | 2 | ||
| HSA concentration/mg/mL | A | 10 | 30 | 50 | 70 | |
| pH value | B | 5.5 | 7 | 8.5 | 10 | 11.5 |
| Volume added ethanol/mL | C | 2.25 | 3 | 3.75 | 4.5 | 5.25 |
| Speed of ethanol addition/mL/min | D | 0.5 | 1.25 | 2 | 2.75 | |