Table 2.
Optimized operating conditions for the determination of sildenafil.
| Parameters | Value/selected characteristic(s) |
|---|---|
| Mobile phase | Methylene chloride-methanol (9 : 1, v/v) |
| Solubilization and dilution solvent | Methanol |
| Plates | Merck TLC silica gel F254 20 × 10 cm |
| Deposited volume on the plate | 5 μL |
| Width of the deposit strip | 7.4 mm |
| Length between deposits | 9.4 mm |
| Distance from the deposition line to the edges of the plate (abscissa and ordinate) | 8 and 10 mm |
| Development distance on plate | 70 mm |
| Development mode | Linear ascending mode |
| Saturation time of mobile phase | 30 min |
| Deposit flow | 15 µL/s |
| Deposit mode | Spray |
| Gas for sample application | Nitrogen |
| Detection wavelength | 305 nm |