Figure 6.

Test of IGF-1 drug identified via gene expression analysis. a) Schematic indicating the culture procedure for the drug testing and shows the endpoint assays (i.e., diffusion assay, permeability and sprout quantification). b) Representative images of control and IGF-1-treated co-culture models. 12 μg/mL of IGF-1 treatment or IgG isotype control was added to the co-cultures. c) Sprout length fold change. d) Number of sprouts fold change. e) Permeability fold change (Mann-Whitney U test after normality was disproved via Kolmogorov-Smirnov test). N = 4 lumens (technical replicates) each from at least 3 independent biological experiments. Bars represent average ± S.E.M. Scale bar = 270 μm *p ≤ 0·05, **p ≤ 0·01, ****p ≤ 0·0001 via Welch-corrected t-test unless specified otherwise.