FIG. 11.

Steady-state level of Nop1p and Qsr1p upon Spb1p depletion. A strain expressing HA-Spb1p under the control of a GAL promoter (YDK14-1A/pDK376) was grown in galactose until early log phase and then transferred to glucose-containing medium to turn off the expression of Spb1p. Samples were taken at different times after the shift, and proteins were extracted. Equal amounts of proteins for each time point were separated by SDS–10% PAGE. Western blotting with anti-HA antibodies detects HA-Spb1p migrating at 120 kDa (upper panel). The anti-Nop1p revealed the protein at 38 kDa (middle panel), and Qsr1p was detected at 20 kDa (lower panel). MW, molecular weight markers in thousands.