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. 2021 Jul 21;14(6):1295–1305. doi: 10.1038/s41385-021-00430-6

Fig. 5. Loss of CISH in ILC2s impairs control of Salmonella infection.

Fig. 5

a Colony forming units (CFU) of S. typhimurium in spleens of indicated mice on day 3 of infection. b CFU from mesenteric lymph nodes (MLN) of indicated mice at day 3. for a and b, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for one-way ANOVA with Dunnett testing for multiple comparisons. n = 7–10 mice/group, pooled from 2 of 3 independent experiments. c Cytokine transcripts from dissociated MLNs of indicated genotype collected on day 3 of infection and cultured overnight. *p < 0.05 by t-test. unannotated comparisons not significant. n = 3 mice/group. d Representative gross appearance and H&E-stained histologic appearance of ceca from indicated strains. Scale bars represent 100 μm. e CFU from MLN of WT (closed circles, white bars) or Pou2f3−/− (open circles, gray bars) mice treated with PBS or IL-25 to activate ILC2s. **p < 0.01 by t-test. n = 4-5 mice/group. f CFU from epithelial-enriched fraction (bulk epi) or sorted CD45– (predominantly epithelial cells) or CD45+ (intraepithelial leukocytes, “iEL”) populations from the epithelial-enriched fraction (left of dotted line); or from the bulk lamina propria-enriched fraction or FACS-sorted CD45+ lamina propria (LP) cells (right of dotted line) of WT or ΔILC2 mice. n.d. not detected. *p < 0.05, **p < 0.01 by t-test. n = 3 mice/group. g mRNA expression of M1 and M2 markers in FACS-sorted lamina propria macrophages from uninfected (naïve) WT (clear bars) or ΔILC2 (gray bars) mice. *p < 0.05. unannotated comparisons not significant. n = 4 mice/group. h FACS-sorted lamina propria macrophages from uninfected (naïve) WT (clear bars) or ΔILC2 (gray bars) were infected in vitro with S. typhimurium, cleared of extracellular bacteria, and lysed to quantify surviving/intracellular bacterial CFUs. *p < 0.05 by t-test. n = 3 mice/group. i CFU of S. typhimurium in spleens of indicated genotypes of mice on day 3 of infection. j CFU from MLN of indicated mice at day 3. for i and j, *p < 0.05 by t-test. n = 6 mice/group.