Fig. 4. Ablation of Prkar2a in immune cells does not protect mice against DSS-induced colitis.
a–e Prkar2afl (n = 10) and Prkar2afl/LysMCre (n = 10) mice were treated with 2% DSS for 1 week followed by 2 days of normal drinking water. a Weight loss was monitored daily and is displayed as the percentage of the initial body weight. b Stool score was measured every day during colitis development. c At day 9, colons were removed and colon lengths were determined. d Histological analysis of distal colon tissues at day 9 of experimental colitis. e Representative images of distal colon at day 9. Scale bar = 50 μm. f The survival curve of Prkar2afl (n = 14) and Prkar2afl/LysMCre (n = 14) mice. Mice were treated with 3% DSS for 1 week and the mortality was monitored over 14 days. Log-rank (Mantel–Cox) test was used to do the analysis. Data shown in a–f are representative of two independent experiments. All graphs show mean ± SEM. Student’s t test (c, d) or two-way ANOVA (a, b) was used to compare experimental groups. ns not significant.