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. 2021 Aug 4;14(6):1282–1294. doi: 10.1038/s41385-021-00426-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Western blot analysis of STAT3 activation in colon tissues collected from Prkar2a^−/− (n = 4) and WT mice (n = 4) at day 6 of DSS-induced colitis. Data are representative of at least three independent experiments. b Immunoblot analysis of the indicated proteins in colon tissues from WT mice at day 0 (n = 2), day 3 (n = 3), and day 6 (n = 4) of DSS-induced colitis. Data are representative of two independent experiments. c Western blot analysis of the indicated proteins in CCD841 cell line after stimulation with F/I. Data are representative of at least three independent experiments. d Immunoblot analysis of the indicated proteins in RKO cells transfected with siRNA against PRKAR2A or a scramble control and then stimulated with recombinant human IL-6 (10 ng/mL) for 30 min. e Immunoblot analysis of the indicated proteins in CCD841 cells transfected with siRNA against PRKAR2A or a scramble control and then stimulated with recombinant human IL-6 (10 ng/mL) for 30 min. f Immunoblot analysis of the indicated proteins in SW480 cells after stable knockdown of PRKAR2A followed by stimulation with recombinant human IL-6 for 30 min. Data shown in d–f are representative of two independent experiments conducted in duplicate. Student’s t test (a, e, f) or two-way ANOVA (b) or one-way ANOVA (d) was used to do the analysis. ***P < 0.001; *P < 0.05. ns not significant.