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. 2021 Aug 4;14(6):1282–1294. doi: 10.1038/s41385-021-00426-2

Fig. 8. Inhibiting ISGs partly reverses the protection against DSS-colitis in Prkar2a−/− mice.

Fig. 8

a RT-qPCR analysis ISGs (IRF7, ISG15, OAS2, USP18, and APOL9A) in colon tissues of Prkar2a−/− mice treated (n = 3) or untreated (n = 3) with TSA. Data are representative of two independent experiments conducted in duplicate. b–e Prkar2a−/− mice (n = 5), TSA-treated Prkar2a−/− mice (n = 5), and WT mice (n = 5) were challenged with 1.5% DSS for 6 days followed by 2 days of normal water to induce experimental colitis. b Weight loss was determined daily and is displayed relative to initial weight. c Histological scores. d Representative H&E-stained images of colon sections. Scale bar = 50 µm. e At day 9, the mice were sacrificed and colons were removed to calculate the length. Data shown in b–e are representative of three independent experiments. Results are presented as mean ± SEM. Student’s t test (a) or one-way ANOVA (c, e) or two-way ANOVA (b) was used to compare the experimental groups. *P < 0.05, **P < 0.01, ***P < 0.001.