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. 2021 Aug 29;473(11):1749–1760. doi: 10.1007/s00424-021-02611-5

Table 2.

Effects of hydrochlorothiazide on urine volume, glomerular filtration rate, ENa, and EK, in mice on the Control-K+ and Low-K+ diet

UV
(μl·min−1
100 g BW−1)		 GFR
(ml·min−1
100 g body BW−1)		 ENa
(μeq·min−1
100 g body BW−1)		 EK
(μeq·min−1
100 g body BW−1)		 ENa + EK
(μeq·min−1
100 g body BW−1)		 EK/ENa + EK ENa/EK
Control-K+ diet
Male mice
Baseline  3.7 ± 0.4 0.72 ± 0.09 0.36 ± 0.1 0.42 ± 0.05 0.78 ± 0.1 0.55 ± 0.07 1.01 ± 0.3
0–30 17.6 ± 2.3* 0.77 ± 0.08 2.39 ± 0.3* 1.00 ± 0.13* 3.40 ± 0.4* 0.31 ± 0.03* 2.44 ± 0.3*
30–60 8.2 ± 1.9* 0.49 ± 0.06 1.38 ± 0.4* 0.48 ± 0.08 1.86 ± 0.4* 0.29 ± 0.04* 2.87 ± 0.5*
60–90 4.8 ± 1.1 0.40 ± 0.05* 0.88 ± 0.3* 0.33 ± 0.07 1.21 ± 0.3 0.31 ± 0.07* 3.13 ± 0.8*
90–120 3.4 ± 0.5 0.36 ± 0.05* 0.63 ± 0.1* 0.31 ± 0.07 0.93 ± 0.2 0.34 ± 0.05* 2.45 ± 0.6*
Female mice
Baseline  3.8 ± 0.6 0.85 ± 0.07 0.49 ± 0.1 0.38 ± 0.05 0.87 ± 0.1 0.47 ± 0.06 1.42 ± 0.4
0–30 32.3 ± 3.3*† 0.97 ± 0.12 5.48 ± 0.8*† 1.73 ± 0.11*† 7.21 ± 0.8*† 0.26 ± 0.03* 3.25 ± 0.5*
30–60 10.1 ± 1.8* 0.67 ± 0.09 1.63 ± 0.6* 1.02 ± 0.24* 2.65 ± 0.7* 0.44 ± 0.06 1.65 ± 0.4
60–90 8.7 ± 2.1 0.49 ± 0.08* 1.11 ± 0.4* 0.76 ± 0.12*† 1.88 ± 0.4 0.46 ± 0.05 1.39 ± 0.3
90–120 6.9 ± 1.1 0.44 ± 0.03* 0.71 ± 0.3 0.65 ± 0.06*† 1.37 ± 0.3 0.57 ± 0.07† 1.11 ± 0.4
Low-K+ diet
Male mice
Baseline  3.7 ± 0.7 1.00 ± 0.14 0.59 ± 0.1 0.07 ± 0.02‡ 0.66 ± 0.1 0.13 ± 0.04‡ 14.16 ± 5.7‡
0–30 21.9 ± 3.9* 1.05 ± 0.09‡ 5.15 ± 0.9*‡ 0.22 ± 0.05*‡ 5.37 ± 1.0* 0.04 ± 0.01‡ 25.38 ± 4.2‡
30–60 18.0 ± 2.3*‡ 0.87 ± 0.05‡ 4.39 ± 0.6*‡ 0.26 ± 0.05*‡ 4.65 ± 0.6*‡ 0.06 ± 0.01‡ 19.63 ± 3.6‡
60–90 13.6 ± 1.4*‡ 0.79 ± 0.04‡ 3.42 ± 0.3*‡ 0.28 ± 0.04* 3.70 ± 0.3*‡ 0.08 ± 0.01‡ 14.09 ± 3.1‡
90–120 11.2 ± 1.2*‡ 0.82 ± 0.09‡ 2.82 ± 0.3*‡ 0.23 ± 0.03* 3.05 ± 0.3*‡ 0.08 ± 0.01‡ 17.24 ± 2.5‡
Female mice
baseline  4.4 ± 0.4 1.10 ± 0.09 1.09 ± 0.2‡ 0.07 ± 0.02‡ 1.16 ± 0.2 0.07 ± 0.03‡ 20.13 ± 4.1‡
0–30 28.9 ± 1.5* 1.14 ± 0.11 6.26 ± 0.6* 0.20 ± 0.06‡ 6.46 ± 0.6* 0.03 ± 0.01‡ 66.13 ± 28.2‡
30–60 25.2 ± 1.3*‡ 0.84 ± 0.09 5.41 ± 0.3*‡ 0.30 ± 0.09*‡ 5.70 ± 0.3*‡ 0.05 ± 0.02‡ 39.00 ± 15.5‡
60–90 18.6 ± 2.3*‡ 0.74 ± 0.02*‡ 3.93 ± 0.5*‡ 0.34 ± 0.09*‡ 4.27 ± 0.4*‡ 0.09 ± 0.03‡ 20.51 ± 7.2‡
90–120 15.2 ± 2.7*‡ 0.77 ± 0.05*‡ 3.30 ± 0.6*‡ 0.27 ± 0.07*‡ 3.57 ± 0.6*‡ 0.09 ± 0.03‡ 18.69 ± 6.3‡

Data are presented as means ± SE; n = 6 mice/group. Control-K+ diet, diet contains 1% KCl; Low-K+ diet, diet contains 0.1% K+; UV, urine volume; GFR, glomerular filtration rate; ENa, absolute excretion of Na+; EK, absolute excretion of K+. ENa + EK, total cation excretion; EK/ENa + EK, fraction of K+ from total cation excretion; ENa/EK, ratio of urine Na+ and K+. ANOVA test was used to analyze significant difference among groups. P < 0.05, significant difference from same group of animals in the control periods (*), between sex in the same diet (†), and between CK and LK in the same sex (‡)