Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 20;12(11):971. doi: 10.1038/s41419-021-04275-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A A schematic diagram outlining the process of osteoclast differentiation from human PBMCs. B B7–H3 mRNA expression during human osteoclast differentiation measured using RT-qPCR. C Whole-cell lysates were immunoblotted with B7–H3, c-Fos, and NFATc1 Abs. D At days 0, 2, and 8, the cell-surface B7–H3 expression was assessed by flow cytometry (solid line: monocytes; dashed line: OCPs or mature osteoclasts; gray shaded: isotype control). E, F OCPs were cultured as described in Fig. 1A. OCPs were incubated with M-CSF (20 ng/ml) in the presence or absence of recombinant human M-CSF receptor/Fc chimera (sM-CSF R) overnight. The expression of B7–H3 and RANK mRNA was measured using RT-qPCR and the expression of B7–H3 protein was detected using Western blot. G, H OCPs were cultured with M-CSF (20 ng/ml) in the presence of RANKL (40 ng/ml) for one or three days. RT-qPCR and Western blot were performed to detect the expression of B7–H3. (B, E, G) mRNA levels were normalized with GAPDH expression.