Skip to main content
. 2021 Oct 20;12:6110. doi: 10.1038/s41467-021-26394-6

Fig. 1. The LNK SH2/JAK2 pY813 co-crystal structure.

Fig. 1

a Schematic representation of the LNK domain architecture. LNK comprises an N-terminal dimerisation domain, a central plekstrin homology domain and a C-terminal SH2 domain. b (left) Cartoon representation of the backbone of the WT LNK SH2 domain with the secondary structural features indicated (peptide not shown), (middle) LNK SH2 domain structure with the JAK2 pY813 peptide shown, and (right) the LNK SH2 domain N-terminal helix extending behind the central β strands, positioned over a hydrophobic surface on the SH2 domain. c Interactions between the LNK SH2 domain (red) and JAK2 pY813 peptide (black). The phosphotyrosine occupies the phosphotyrosine binding pocket of the SH2 domain and is coordinated by R364, S368, R343, and the nitrogen of E367 of LNK. The +1 Glu forms a salt bridge with K384 of LNK and the +3 Leu sits in a hydrophobic pocket formed in part by the EF and BG loops of LNK. d Specific interactions between the LNK SH2 domain and the phosphotyrosine, +1 Glu, and +3 Leu residues highlighted.