Fig. 4. Overexpression of mCAT delays renal failure and mortality in RC/null mice.

a Huge RC/null gross pathology of kidneys from 24-days-old WT and RC/null mice, and RC/null mice treated with AAV9-mCAT (2 × 10⁹/g) (representative image shown; n = 4−6). b Kidney weight normalized to body weight in RC/WT or RC/null mice treated with AAV9 vector alone (AAV9-vector) or AAV9-mCAT. c BUN (mg/dL) and d hemoglobin (g/dL) in RC/WT mice or RC/null mice treated with AAV9 vector alone (AAV9-vector) or AAV9-mCAT; n = 3−7. e Representative PAS staining of kidney sections from RC/null mice, untreated and treated with AAV9-mCAT. f−g Trichrome staining of kidney section from RC/null mice untreated and treated with AAV9-mCAT. h−j Quantitative analysis of cysts area (g), fibrotic area (h), and combined cysts and fibrotic area (i). *p < 0.05.; n = 4 for WT; n = 15−18 for each of the other groups. k Survival curves of RC/null mice untreated or treated with AAV9-mCAT (2*10⁹ /g); n = 31 for RC/null, n = 19 for RC/null+AAV9-mCAT.