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. 2021 Oct 20;11:20711. doi: 10.1038/s41598-021-00098-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Decay time of donor lifetime signals following UV-uncaging of cAMP in cells treated with siRNAs for the indicated PDEs. Note significantly slower breakdown upon knockdown of PDE3A, and a smaller, but still significant contribution of PDE10A. Datapoints are fitted decay times of single cells. For each condition, the experiment was performed in duplicates, with cells grown, transfected, and assayed in two independent wells. Indicated are median value (vertical black line), mean value (green dotted line); boxes encompass middle 50% of values and whiskers represent 1.5 times the interquartile range. Inset: 10 representative traces for cAMP decay in Hela cells with PDE3A gene knock-down (upper panel) and control (lower panel) cells. Cells were imaged every 2 s; uncaging at 25 s using a 200 ms flash of UV light.