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. 2021 May 12;15(11):3159–3180. doi: 10.1038/s41396-021-00992-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Maximum-likelihood tree of concatenated protein sequences from MAGs and genomes. Single marker genes were retrieved with CheckM. Highlighted in blue are MAGs obtained in this study. Highlighted in purple are dsrAB-containing MAGs obtained from the NCBI database from the class Thermoanaerobaculia. The genus Ca. Acidiflorens is represented by the most complete MAG (GCA_003166525.1) from the corresponding study [12]. Our phylogenomic analysis showed that one MAG that was previously assigned to Ca. Aminicenantes (GCA_004524955.1), recovered from the Bothnian Sea [146], is affiliated with the newly proposed family Ca. Sulfomarinibacteraceae. Black dots indicate dsrAB-containing genomes/MAGs. Bootstrap values with >90% are indicated with filled black circles on nodes. Nitrospina gracilis 3/211 (GCA 000341545.2) was used as an outgroup. The scale bar represents 10% sequence divergence.