Fig. 5. Box plots depicting the expression of otr and dsrB relative to a housekeeping gene (DNA-directed RNA polymerase, alpha subunit) from Ca. Sulfomarinibacter kjeldsenii MAG AM3-C during microcosm experiments with amendments of tetrathionate versus no-amendment controls.

Relative expression was determined by rt-qPCR. Expression of otr was significantly higher at day 8 (p = 0.0488) as determined using a two-tailed T-test, and is indicated by an asterisk. Center lines indicate medians; box limits indicate 25th and 75th percentiles as determined by R software; and whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles.