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. 2021 Oct 20;12(11):975. doi: 10.1038/s41419-021-04212-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Immunoblotting for cleaved-PARP and PARP expression in indicated colorectal cancer (CRC) cells under glucose deprivation. GAPDH is used as a loading control. B Schematic diagram of library screening under glucose deprivation. C Venn diagram shows the overlap between genes downregulated by glucose deprivation treatment (n = 2798), and genes targeted by sgRNAs that enriched in Group R (n = 262). D, E RT-qPCR of EZH2 levels in HCT116 and SW480 cells treated with indicated doses of glucose for 24 h (D) or under glucose deprivation for the indicated time (E). 1 × glucose, 25 mM. F Immunoblotting for EZH2 expression in indicated CRC cells under glucose deprivation. Actin is used as a loading control. The experiments in A, F were repeated twice independently with similar results. In D, E, data are mean ± s.d., n = 3 independent experiments; P-values were calculated using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.