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. 2021 Oct 20;12(11):975. doi: 10.1038/s41419-021-04212-7

Fig. 4. GLS is a direct target gene of EZH2 in colorectal cancer cells.

Fig. 4

A–C Scatter plots showing the correlation between EZH2 and GLS mRNA levels in human colorectal cancer (GSE24514, GSE14333, and GSE106582). Statistical analysis was performed using a two-tailed Student’s t-test. r, Pearson’s correlation coefficient. D Schematic representation of the binding site of human EZH2 in GLS promoter as mapped in ENCODE database (ENCSR069DPL). E Schematic representation of the binding site of mouse EZH2 in Gls promoter of primary colon epithelial cells isolated from wild-type mice (GSE84858). F, G Schematic representation of the binding of H3K27ac (F) and H3K27me3 (G) on the GLS gene locus in HCT116 cells with the ENCODE project. H, I WB and RT-qPCR of GLS levels in CRCs pretreated with DMSO or HDAC inhibitor (TSA, 4 μM), followed by glucose deprivation treatment (0 mM, 16 h). J Location of ChIP-qPCR primers on GLS promoter. K, L ChIP-qPCR analysis of EZH2 and H3K27Ac on GLS promoter in HCT116 cells with or without glucose deprivation treatment (0 mM, 12 h). In I, K, and L, data are mean ± s.d., n = 3 independent experiments; P-values were calculated using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001; ns, non-significant.