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. 2021 Oct 20;12:6101. doi: 10.1038/s41467-021-26331-7

Fig. 8. gakh-1 regulates basal mitophagy in vivo.

Fig. 8

a Schematic representation of GAK domain structure and orthologues gakh-1 and dnj-25 present in C. elegans. Homology values were obtained by protein blast alignment. b In vivo detection of mitophagy in C. elegans. Transgenic nematodes expressing mtRosella in body-wall muscle cells were treated with gakh-1 RNAi or pL4440 control vector. dsRED only structures represent mitochondria in acidic compartments (arrowheads). Scale bar = 50 μm, insets = 5 µm. Representative image from n = 25 worms. c Mitophagy stimulation signified by the ratio between pH-sensitive GFP to pH-insensitive dsRED (n = 25 worms per group, upper panel). Quantification of the frequency of mitochondria undergoing mitophagy (dsRED puncta lacking EGFP co-localisation) are expressed as a percentage of total mitochondria detected (n = 25 worms per group, lower panel). The data are presented as violin plots of individual values with median (red, solid line) and quartiles (red, dashed line) shown. Significance was determined by unpaired two-tailed t test from n = 2 independent experiments, where **P < 0.001 or ****P < 0.0001. For precise P values, see the source data file.