Table 6.

Effect of organic acids on mRNA gene expression in the jejunum and cecal tonsil of broilers with and without coccidial challenge

Treatments1	C.C2	Jejunum3						Cecal Tonsil3
		18d			29d			18d			29d
		CLDN1	ZO-1	OCLN	CLDN1	ZO-1	OCLN	CLDN1	ZO-1	OCLN	CLDN1	ZO-1	OCLN
CON	−	1.06	1.14	1.29	1.32	1.14	1.06	1.02	1.09	1.13	1.04	1.01	1.05
OAW	−	1.49	2.43	3	1.89	1.4	1.81	2.3	1.45	1.34	1.31	1.41	1.23
OAF	−	1.11	1.06	0.72	1.94	1.93	4.03	1.94	2	1.96	0.61	1.04	1.27
CONC	+	1.22	1.24	1.13	0.92	1.22	2.39	1.1	1.35	2.39	0.38	0.71	1.51
OAWC	+	2.04	1.04	0.8	0.97	2.02	4.31	1.52	1.73	1.47	0.83	1.22	1.24
OAFC	+	2.13	1.26	1.43	3.41	0.98	2.66	1.06	1.49	1.52	0.69	0.92	0.87
SEM		0.65	0.591	0.836	0.775	0.365	1.538	0.557	0.304	0.337	0.26	0.225	0.221
Main effect means
CON		1.14	1.19	1.21	1.12	1.18	1.73	1.06	1.22	1.76	0.71	0.86	1.28
OAW		1.77	1.73	1.9	1.43	1.71	3.06	1.91	1.59	1.41	1.07	1.32	1.23
OAF		1.62	1.16	1.08	2.67	1.45	3.34	1.5	1.75	1.74	0.65	0.98	1.07
C.C	−	1.22	1.54	1.67	1.72	1.49	2.3	1.75	1.52	1.48	0.99	1.15	1.18
	+	1.8	1.18	1.12	1.77	1.41	3.12	1.23	1.52	1.79	0.63	0.95	1.21
P-value	Diet	0.61	0.559	0.58	0.128	0.365	0.54	0.331	0.227	0.516	0.235	0.13	0.623
	C.C	0.288	0.459	0.432	0.939	0.791	0.519	0.259	0.977	0.262	0.109	0.28	0.901
	Diet × C.C	0.802	0.338	0.223	0.287	0.114	0.446	0.641	0.357	0.054	0.358	0.926	0.167

¹CON Control diet, OAW Organic acid in water, OAF Organic acid in feed, CONC Control diet with Coccidial challenge, OAWC Organic acid in water with Coccidial challenge; and OAFC Organic acid in feed with Coccidial challenge

²C.C = Coccidial Challenge

³CLDN1 = Claudin1; ZO-1 = Zona Occludin-1; OCLN = Occludin