Figure 3.

gHA immunogens elicit cross-immunogen reactivity. Plot titles indicate immunogen and x-axis indicates Luminex antigen. At day 7 (d7), gHA^RBSv2.0 (n=6) (A) and gHA^Shield (n=5) (B) immunogens elicit interface-directed serum responses, while the cysteine-stabilized counterpart immunogens (C, D) elicit a nearly undetectable serum response. By day 28 (d28, right), cysteine-stabilized gHA_cys ^RBSv2.0 (n=5) (C) and gHA_cys ^shield (n=5) (D) immunogens elicit a weak, but present serum response to all immunogens. All serum responses are normalized to a control antibody, FL-1086, that binds all four immunogens, and reported as ng/mL FL-1086 msIgG1 equivalents ± SD; n = number of mice used. Statistical significance was determined by a student’s t-test with Welch’s correction. (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.