Figure 5.

Hypoxia attenuates DNA methyltransferase (DNMT) activity in human bronchial epithelial cells. The human bronchial epithelial cell line 16HBE140- was cultured and exposed to 1 or 21% oxygen for 24 or 48 h. Similarly, primary epithelial cells cultured from small airway brushes were exposed to 1 or 21% oxygen for 48 h. DNMT activity was measured and normalized to the normoxia group mean values. Hypoxia and normoxia values were compared in 16HBE140- cells using the Wilcoxon rank sum test and in primary cells using the Wilcoxon signed rank test (paired by subject identifier).