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. 2021 Oct 7;12:741342. doi: 10.3389/fpls.2021.741342

FIGURE 4.

FIGURE 4

A Valsa mali effector protein 1 suppresses MdPR10-mediated callose deposition. (A) Aniline blue staining with leaves at 48 h after expression of MdPR10 or co-expression of MdPR10 and VmEP1 in the apple leaves. Bars = 100 μm. The expression of EV as control. Bars = 50 μm. (B) Quantification of the callose deposition in apple leaf tissues, as determined by ImageJ software. Mean ± SD; n > 10; a, b, and c indicate t-test P < 0.05; Duncan’s multiple range test. (C) The mRNA level of MdCalS5 in the apple plantlets, as revealed by qRT-PCR 2 days after infiltration of OE-MdPR10. The transcript levels were analyzed by qRT-PCR and normalized to that in the EV using the EF-1α gene as an internal reference. Mean ± SD; n = 3; a, b, and c indicate t-test P < 0.05; Duncan’s multiple range test. These experiments were repeated three times with similar results. Panels (A–C) EV, pCAMBIA1302 empty vector.