Skip to main content
. 2021 Oct 7;12:741342. doi: 10.3389/fpls.2021.741342

FIGURE 5.

FIGURE 5

A Valsa mali effector protein 1 attenuates the MdPR10-mediated resistance to V. mali. (A) Representative disease symptoms of the apple leaves overexpressing the genes at 36 hpi of V. mali. Bars = 5 mm. (B) The average lesion diameter in the apple leaves in which genes overexpression was evaluated at 36 hpi of V. mali. Means ± SDs; n > 15; a and b indicate t-test P < 0.05; Duncan’s multiple range test. (C) V. mali biomass was measured with qPCR at 36 hpi of V. mali. The relative V. mali biomasses were normalized to the mean of the control. Means ± SDs; n = 3; a and b indicate t-test P < 0.05; Duncan’s multiple range test. The genome DNA of apple leaves was extracted and the relative V. mali biomass was estimated by DNA-based quantitative PCR (qPCR). These experiments were repeated three times with similar results. (D) Immunoblot analysis of the expressed proteins were performed with anti-GFP antibody. CBB staining (bottom) was used as a loading control.