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. 2021 Oct 7;11:756205. doi: 10.3389/fonc.2021.756205

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Copyright © 2021 Li, Zhao, Zhang, Tao, Shen, Li, Ma, Li and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of exosomes on HCC cell growth. (A) Proliferation of PLC/PRF/5 and MHCC-97H in the presence of various concentrations of exosomes isolated from culture medium of the same cell line as measured by CCK8 assay. (B) Colony formation assay and (C) count of sphere formed in PLC/PRF/5 and MHCC-97H with and without exosomes (10 μg/ml) isolated from the culture medium of the same cell line. (D) Quantitative PCR analysis of the mRNA levels, and (E) Western blot analysis of the protein levels of stem cell marker genes in PLC/PRF/5 and MHCC-97H cells cultured with and without exosomes (10 μg/ml) isolated from the culture medium of the same cell line. The data are presented as an average of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001.