Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 7;11:756205. doi: 10.3389/fonc.2021.756205

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Li, Zhao, Zhang, Tao, Shen, Li, Ma, Li and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The effect of exosomes isolated from Shh knockdown cells. (A) Western blot analysis on the transfection of Shh shRNA lentivirus on the expression of cellular Shh and Exo Shh. (B) Quantitative PCR analysis of the mRNA levels, and (C) Western blot analysis of the protein levels of hedgehog signaling pathway target genes in PLC/PRF/5 and MHCC-97H cells cultured with exosomes isolated from the culture medium of the same cell line that were transfected with vector or shRNA containing lentivirus. (D) Cell proliferations as measured by CCK8 assay, (E) colony formation assay, and (F) count of sphere formed in PLC/PRF/5 and MHCC-97H cells cultured with exosomes isolated from the culture medium of the same cell line that were transfected with vector or shRNA containing lentivirus. The data are presented as an average of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001.