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. 2021 Oct 7;8:698698. doi: 10.3389/fmolb.2021.698698

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Copyright © 2021 Hong, Zha, Wang, Qiao and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of folic acid on the pyroptosis and Hippo signaling pathway. (A) Representative immunoblot showing expression levels of NLRP3, ACS, Cleaved-Caspase-1 and Caspase-1. (B) Quantification of NLRP3, ACS, Cleaved-Caspase-1 and Caspase-1 with α-tubulin as the loading control. (C) IL-1β and IL-18 levels in the cell supernatant. (D) Representative immunoblot showing expression levels of YAP1 and p-YAP1. (E) Quantification of YAP1 and p-YAP1 with α-tubulin as the loading control. ^* p < 0.05, compared with NG; ^# p < 0.05, compared with HGF; ^& p < 0.05, compared with HGF+50 nM folic acid.